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>>Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City

September 22, 2014

Posted under Lab Test

U.P. Lab Tests show that Boston C kills cancer cells within 3-5days

These are the results of the 2002 – 2003 laboratory tests conducted by the U.P. Institute of Biology using Boston C on breast cancer, leukemia, bladder carcenoma, jukrat cell pymphoma, lung & colon cancer.

Likewise, Dr. Gil Vicente, and ENT from St. Luke’s Hospital tested Boston C on lung and cervical cancer for presentation to the World Health Organization. The results below show that even in its very dilutedform, BostonC is still able to kill cancer cells.


 November 3,2002

RESULTS OF THE CYTOTOXICITY ASSAY ON BOSTON C ON HUMAN LUNG AND COLON CARCINOMA

Assay procedure:

The product Boston A was tested for cytotoxicity on two human cancer cell lines — non small cell lung adenocarcinoma A549 and colon carcinoma from the American Type Culture Collection (ATCC) at the Mammalian Cell Culture Laboratory of the Institute of Biology, U.P. Diliman, Quezon City. The MTT cytotoxicity assay used is based on the ability of live cells to reduce the substance MTT into formazan crystal which when dissolved in the solvent dimethyl sulfoxide (DMSO) will result into a purple solution. The cells were seeded at 10,000 cell per well into 96 well mcirotitier plates and subsequently treated with different dilutions of the product. Incubation of the cells with the product for 72 hours followed thereafter. Negative controls were cells with no treatment whole positive controls were cells treated with routinely used chemotherapeutic drugs Vincristine or Doxorubicin. Concentration that inhibited the growth of cells at 50% (IC50) were computed. Substances with low IC50 indicate potential for cytotoxicity.

With Boston A, since the product is a solution whose active principle dissolved wiothin the liquid is not know, the IC50 was computed as percentage of the product dissolved in the media over the volume v/v.

The results are as follows: IC50
Boston A on A549 (lung carcinoma)……0.078
Boston A on colon carcinoma………………0.625


 9 December 2003

RESULTS OF THE CYTOTOXICITY ASSAY ON BOSTON C ON HL60 LEUKEMIA CELL USING TRYPAN BLUE EXCLUSION ASSAY

Assay procedure:

Boston C, a liquid product was brought to the Mammalian Cell Culture Laboratory of the Institute of Biology, U.P. Diliman for testing for cytotoxicity against HL60, a leukemia cell line (ATCC). Since the product was a mixture of unknown compounds, it was tested in different dilutions and the percentage concentration which showed 50% inhibition of growth (IC52) of the cells was computed. Since the cell line is maintained as a suspension culture. Trypan blue exclusion assay and direct cell counting were done. Cells were incubated with the extract for 5 days at 10,000 cells per well in a 96 well plate. After 3 and 5 days, live cells which did not take in stain and dead cells which took in stain were counted. Percentage of dead cells over the total number of cells were computed. These data were used in the computation of the IC50. Cells treated with dimethyl sulfoxide (DMSO), the solvent used for the extract was the negative control.

Results:
After 3 days: MO IC50 could be computed.
After 5 days: IC50=0.175%


 9 December 2003

RESULTS OF THE CYTOTOXICITY ASSAY ON BOSTON ‘C’ ON JUKRAT CELL LYMPHOMA USING TRYPAN BLUE EXCLUSION ASSAY

Assay procedure:

Boston C, a liquid product was brought to the Mammalian Cell Culture Laboratory of the Institute of Biology U.P. Diliman for testing for cytotoxcity against Jukrat, a lymphoma cell line (ATCC). Since the product was a mixture of unknown compounds, it was tested in different dilutions and the percentage concentration which showed 50% inhibition of growth (IC50) of the cells was computed. Since the cell line is maintained as a suspension culture, Trypan blue exclusion assay and direct cell counting were done. Cells were incubated with the extract for 5 days at 10,000 cells per well in a 96 well plate. After 3 and 5 days, live cells which did not take in stain and dead cells which took in stain were counted. Percentage of dead cells over the total number of cells were computed. these data were used in the computation of the IC50. cells treated with dimethyl sulfoxide (DMSO) the solvent used for the extract was the negative control.

Results:
After 3 days: no IC50 could be computed
After 5 days: IC50-0.625%


19 December 2003

RESULTS OF THE CYTOTOXICITY ASSAY ON BOSTON C ON MCF7 BREAST CARCINOMA USING MTT

Assay procedure:

Boston C, a liquid product was brought to the Mammalian Cell Culture Laboratory of the Institute of Biology U.P. Diliman for testing for cytotoxcity against MCF7, a human breast carcinoma cell line (ATTC). Since the product was a mixture of unknown compounds, it was tested in different dilutions and the percentage concentration which showed 50% inhibition of growth (IC50) of the cells was computed. The cell line is maintained as an adherent culture. The cells at 10,000 per well were incubated with different dilutions of the extracts in a 96 well plate for 72 hours. Thereafter, MTT was added and the cells were incubated further for 4 hours. Dimenthyl sulfoxide (DMSO) was added and the color changed based on the number of live cells was read at 570nm with an ELISA plate reader. Cells treated with DMSO, the solvent used for the extract, was the negative control.

Results: IC50=0.175%


 19 December 2003

RESULTS OF THE CYTOTOXICITY ASSAY ON BOSTON ‘C’ ON T24 HUMAN BLADDER CARCINOMA USING MTT

Assay procedure:

Boston C, a liquid product was brought to the Mammalian Cell Culture Laboratory of the Institute of Biology U.P. Diliman for testing for cytotoxcity against T24, a human bladder carcinoma cell line (ATCC). Since the product was a mixture of unknown compounds, it was tested in different dilutions and the percentage concentration which showed 50% inhibition of growth (IC50) of the cells was computed. The cell line is maintained as an adherent culture, the cells at 10,000 per well were incubated with different dilutions of the extracts in a 96 well late for 72 hours. Thereafter, MTT was added and the cells were incubated further for 4hours. Dimethyl sulfoxide (DMSO) was added and the color change based on the number of live cells was read at 570 NM with an ELISA plate reader. Cells treated with DMSO, the solvent used for the extract, was the negative control.

Results: IC50+0.325%



Summary of the Laboratory Test Results
of the Cytotoxicity Assay on Boston C

Type of Cancer
Dilution %
After 3 days
After 5 Days

Lung
0.078%
IC50
 
Colon
0.625%
IC50
 
Breast
0.175%
IC50
 
Bladder
0.325%
IC50
 
Cervix
0.128%
IC68
 
Leukemia
0.175%
 
IC50
 

 

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